SubtiBank SubtiBank
Version comparison:

2017-10-15 16:20:522025-05-11 07:31:14

locus

BSU15930

BSU_15930

geneLength

747

750

function

cleaves both 5- and 3-sites of the small cytoplasmic RNA precursor (scr)

processing and degradation of RNA molecules

outlinks

bsu

BSU15930

BSU_15930

Gene

Phenotypes of a mutant

essential [Pubmed|23300471,12682299,11123676]

the ''[[gene|rnc]]'' gene can be deleted in strains cured of the [SW|Skin element] and [[SPß prophage]] (or upon deletion of the toxin genes ''[[gene|txpA]]'' and ''[[gene|yonT]]'') [Pubmed|23300471]

essential [Pubmed|23300471,12682299,11123676]

the ''[[gene|rnc]]'' gene can be deleted in strains cured of the [SW|Skin element] and [SW|SP-beta prophage] (or upon deletion of the toxin genes ''[[gene|txpA]]'' and ''[[gene|yonT]]'') [Pubmed|23300471]

The protein

Catalyzed reaction/ biological activity

Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)

cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[gene|scr]]'')

degradation of ''[[gene|txpA]]'' mRNA-[[protein|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0]] RNA duplex (encoded on the [SW|Skin element]) [Pubmed|23300471]

degradation of ''[[gene|yonT]]'' mRNA-''[[gene|SR6]]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]

degradation of the ''[[gene|roxS]]''-''[[gene|ytdI]]'' and ''[[gene|roxS]]-[[gene|sucC]]'' RNA duplexes [Pubmed|25643072]

Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)

cleaves 16S rRNA 2 nts downstream of the 3' end [PubMed|26883633]

cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[gene|scr]]'')

degradation of ''[[gene|txpA]]'' mRNA-[[protein|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0]] RNA duplex (encoded on the [SW|Skin element]) [Pubmed|23300471]

degradation of ''[[gene|yonT]]'' mRNA-''[[gene|SR6]]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]

degradation of the ''[[gene|roxS]]''-''[[gene|ytdI]]'' and ''[[gene|roxS]]-[[gene|sucC]]'' RNA duplexes [Pubmed|25643072]

Labs working on this gene/protein

[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]

[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]

References

Reviews

17194582, 16179796, 8936814, 16855311, 23300472, 25292357, 25808661, 25878039

17194582, 16179796, 8936814, 16855311, 23300472, 25292357, 25808661, 25878039, 29651979, 31464530

Gene

additional information

[SW|RNase III targets| RNAs that are targeted by RNase III] [pubmed|26883633]

The protein

Protein family

ribonuclease III family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

RNase III domain (aa 20-149) (according to UniProt)

DRBM domain (aa 175-222) (according to UniProt)

Expression and Regulation

additional information

the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]

labs

[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]

[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]