2017-10-15 16:20:522025-05-11 07:31:14
locus
BSU15930
BSU_15930
geneLength
747
750
function
cleaves both 5- and 3-sites of the small cytoplasmic RNA precursor (scr)
processing and degradation of RNA molecules
outlinks
bsu
BSU15930
BSU_15930
Gene
Phenotypes of a mutant
essential [Pubmed|23300471,12682299,11123676]
the ''[[gene|rnc]]'' gene can be deleted in strains cured of the [SW|Skin element] and [[SPß prophage]] (or upon deletion of the toxin genes ''[[gene|txpA]]'' and ''[[gene|yonT]]'') [Pubmed|23300471]
essential [Pubmed|23300471,12682299,11123676]
the ''[[gene|rnc]]'' gene can be deleted in strains cured of the [SW|Skin element] and [SW|SP-beta prophage] (or upon deletion of the toxin genes ''[[gene|txpA]]'' and ''[[gene|yonT]]'') [Pubmed|23300471]
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[gene|scr]]'')
degradation of ''[[gene|txpA]]'' mRNA-[[protein|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0]] RNA duplex (encoded on the [SW|Skin element]) [Pubmed|23300471]
degradation of ''[[gene|yonT]]'' mRNA-''[[gene|SR6]]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]
degradation of the ''[[gene|roxS]]''-''[[gene|ytdI]]'' and ''[[gene|roxS]]-[[gene|sucC]]'' RNA duplexes [Pubmed|25643072]
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
cleaves 16S rRNA 2 nts downstream of the 3' end [PubMed|26883633]
cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[gene|scr]]'')
degradation of ''[[gene|txpA]]'' mRNA-[[protein|FFCE5AD9E04E6D5F7970E89781C545E7DBDF45F0]] RNA duplex (encoded on the [SW|Skin element]) [Pubmed|23300471]
degradation of ''[[gene|yonT]]'' mRNA-''[[gene|SR6]]'' RNA duplex (encoded on the [[SP-beta prophage]]) [Pubmed|23300471]
degradation of the ''[[gene|roxS]]''-''[[gene|ytdI]]'' and ''[[gene|roxS]]-[[gene|sucC]]'' RNA duplexes [Pubmed|25643072]
Labs working on this gene/protein
[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
Gene
additional information
[SW|RNase III targets| RNAs that are targeted by RNase III] [pubmed|26883633]
The protein
Protein family
ribonuclease III family (single member, according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
RNase III domain (aa 20-149) (according to UniProt)
DRBM domain (aa 175-222) (according to UniProt)
Expression and Regulation
additional information
the mRNA is cleaved by [[protein|Rnc|RNase III]] [PubMed|26883633]
labs
[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]